Proteomics - National Center for Research Resources
Search NCRR

Software and Tools

PepAligner

The PepAligner program can read a file containing peptides and align them to a file of protein sequences (.Fasta or delimited text) using Smith-Waterman alignment. The output file will show the original peptide along with the regions of proteins that align well with the peptide (see below for examples).

Please see the Command Line Application Help page for additional information on running this program at the Windows command prompt.

Download Software Tool Download Source Code  

Version v1.0.2895 Requirements Microsoft NET Framework 1.1
Date Updated December 5, 2007 File Size (Software Tool) 173 KB (ZIP)
Registration Required No File size (Source Code) 659 KB (ZIP)
Developers Matthew Monroe
Comments See the complete Revision History for a history of changes


Example Data Files

Input Peptides

  LRDKLQLVGDDLFVTNTK
  MQQQCATLADLAVGTAAGQLK
  WTSAVLGAAGLGGQALALLLK
  WWFMREHWLLACEDSSLEEAKR
  FNNAAACTLADLAVGTAAGKLK
  VVVLGGGDTAMDMKR
  WRWRRNWYWHEFESRDTYWR
  WLDAVTAAGVEVAK
  WHWHWYHHPENLYNNGKTPDR
  WWWWFVTPWFGMSGTLLYMTR
  WWYVTADGFCSSGDGTDWSTYK
		

Output using a Shewanella .Fasta file

PepAligner Output Alignment File

 

Detailed Example Input and Output Files


 

Acknowledgment

All publications that result from the use of this software should include this statement:

Portions of this research were supported by the W.R. Wiley Environmental Molecular Science Laboratory (a national scientific user facility sponsored by the U.S. Department of Energy's Office of Biological and Environmental Research and located at PNNL). PNNL is operated by Battelle Memorial Institute for the U.S. Department of Energy under contract DE-AC05-76RL0 1830.